Wyatt Technology WH-03 Multi-Angle Light Scattering Detector

Description:

The Wyatt WH-03 Heleos II DAWN 8 is an advanced HPLC multi-angle light scattering (MALS) detector designed for absolute molecular weight, size, and conformation analysis of proteins, polymers, nanoparticles, and macromolecules in solution. Commonly used in biopharmaceutical research, polymer science, protein characterization, SEC/GPC analysis, and advanced laboratory chromatography systems where highly accurate molecular characterization is required. Scientists and analytical chemists use the DAWN HELEOS II for SEC-MALS and HPLC-based absolute molar mass measurements without column calibration standards.

Applications:

The Wyatt Heleos II DAWN 8 detector is used in analytical laboratory environments where proteins, polymers, conjugates, nanoparticles, and biopolymers must be characterized for molecular weight, size, aggregation state, and structural conformation. It is commonly integrated with HPLC, SEC, GPC, and FFF systems in pharmaceutical development, biotechnology, polymer analysis, and nanotechnology research laboratories. The DAWN platform provides absolute molar mass determination independent of chromatographic calibration standards.

Technical Specifications:

• Manufacturer: Wyatt Technology
• Model / Part Number: WH-03 / Heleos II DAWN 8
• Product Type: Multi-Angle Light Scattering (MALS) Detector
• Detection Method: Static multi-angle light scattering
• Application: HPLC / SEC-MALS / GPC molecular characterization
• Detector Angles: 8-angle or HELEOS II multi-angle optical configuration
• Molecular Weight Range: Approx. 200 Da to 1 GDa depending on configuration
• Molecular Size Range: Approx. 10 nm to 500 nm radius of gyration measurement
• Laser Type: High-stability laser light source
• Standard Laser Wavelength: 658 nm typical
• Software Compatibility: ASTRA analytical software platform
• Supported Techniques: SEC-MALS, GPC-MALS, FFF-MALS, batch light scattering
• Compatible Systems: HPLC, GPC, SEC, FPLC, and field-flow fractionation systems
• Measurement Capability: Absolute molar mass, particle size, aggregation, branching, conjugation analysis
• Construction: Precision laboratory optical detection instrument
• Application Environment: Pharmaceutical, biotechnology, polymer, and nanotechnology laboratories

Common Replacements:

• Wyatt DAWN 8+ MALS Detector
• Wyatt miniDAWN
• Waters multi-angle light scattering detectors